Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 2;217(4):1319–1334. doi: 10.1083/jcb.201708179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Yue et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Defective chemomechanical coupling of the kinesin-4 motors KIF27 and KIF7. (A–C) Truncated dimeric motors versions of the kinesin-1 KIF5C(1–560) or the kinesin-4s KIF27(1–370)-LZ or KIF7(1–558) were tagged at their C termini with 3×mCit. Cell lysates expressing equivalent amounts of motors were added to flow cells containing taxol-stabilized microtubules in the presence of the indicated nucleotides. (A) Representative images of motor (green) binding to microtubules in the presence of ADP, AMPPNP, or apyrase. Bar, 5 µm. (B) The fluorescence intensity of each motor along microtubules was quantified for each nucleotide condition. The mean fluorescence intensity of each motor in AMPPNP and apyrase was normalized to the mean fluorescence intensity in the ADP state. ***, P < 0.001 as compared with the ADP state (two-tailed t test). (C) The fluorescence intensity of each motor in the presence of ADP was compared with that of the kinesin-1 KIF5C. ***, P < 0.001 as compared with KIF5C (two-tailed t test). Data indicate means ± SEM of more than five microtubules from one representative experiment.