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. 2018 Apr 2;217(4):1303–1318. doi: 10.1083/jcb.201709007

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© 2018 Niewidok et al.

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Figure 7. — Reduction in the number of LC regions of G3BP1 results in less detectable mobile molecules within the liquid phase without changing binding properties to nanocores. (A) Example of a representative granule after expression of a deletion fragment of G3BP1 (G3BP1_C) lacking two LC domains fused to HaloTag on a SNAP-IMP1 background. The granule was identified based on the SiR staining (left) and localizations and trajectories based on TMR staining of HaloTag-G3BP1_C (right) are shown. The border of the SG is indicated by a dashed line. Note that most of the trajectories remained locally very restricted. Bar, 1 µm. (B) Probability density of single-molecule diffusion coefficients from trajectories of a granule expressing HaloTag-G3BP1_C showing the presence of a substantial fraction of essentially immobile molecules. (C) Lifetime determination of HaloTag-G3BP1_C on SNAP-IMP1 background in the bound fraction of SGs. Quantification of granules from six cells from two independent experiments was performed. For all experiments, stress had been induced by a 20-min treatment with 0.5 mM sodium arsenite before imaging.