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. 2018 Apr 2;217(4):1521–1536. doi: 10.1083/jcb.201709121

Figure 4.

Figure 4.

RPA-SIRF comparison with IF. (A) Representative images of IF staining for RPA (red) and DAPI (blue) in untreated HAP-1 cells and cells treated with HU or camptothecin (CPT) for 4 h, as indicated. Top: DNA fork structures reported for the corresponding treatments. (B) Representative images of RPA-SIRF in HAP-1 cells treated with EdU or EdU followed by HU for 4 h, as indicated. Top: DNA fork structures reported for the corresponding treatments. Green circles represent incorporated EdU. (C) Column bar graph of percentage of cells containing IF RPA foci with different treatment conditions as indicated, corresponding to Fig. 3 A. nt, not treataed. Error bars represent SEM of combined data from two biological experiments. A minimum of six image fields was acquired for each condition and for each experiment. (D) Scatter plot of RPA-SIRF signals in unperturbed HAP-1 cells and cells treated with EdU followed by HU, as indicated. Bars represent the mean and SD of combined data from repeated experiments. The significance values are derived from Mann-Whitney statistical analysis after normalization to the corresponding EdU-SIRF.