Table 2.
Polar interactions between FMN and amino acids
| FMN | Distance (Å) | Amino acid | ||
|---|---|---|---|---|
| Atom | Atom | Residue | Difference in B factora | |
| O3P | 2.90 ± 0.01 | N | Gly272 | −2.17 |
| 3.43 ± 0.03 | N | Thr273 | −2.68 | |
| O1P | 2.79 ± 0.03 | N | Gly251 | −0.93 |
| 2.75 ± 0.05 | N | Gly223 | −1.24 | |
| O2P | 2.82 ± 0.02 | N | Thr273 | −2.68 |
| 2.73 ± 0.03 | OG1 | |||
| O3′ | 3.39 ± 0.01 | SG | Cys249 | −1.23 |
| 2.94 ± 0.02 | O | Val194 | −1.92 | |
| 3.26 ± 0.05 | NZ | Lys165 | (−0.70) | |
| O2′ | 2.83 ± 0.04 | O | Ala20 | −2.70 |
| 2.93 ± 0.05 | NZ | Lys165 | (−0.70) | |
| N10 | 3.37 ± 0.03 | O | Ala20 | −2.70 |
| N5 | 3.22 ± 0.09 | NZ | Lys44 | (0.17) |
| N1 | 3.30 ± 0.03 | O | Ala20 | −2.70 |
| 3.20 ± 0.03 | NZ | Lys165 | (−0.70) | |
| O4 | 2.76 ± 0.05 | NZ | Lys44 | (0.17) |
| 3.49 ± 0.04 | N | Ser45 | (0.17) | |
| 3.32 ± 0.04 | OG | |||
| N3 | 2.66 ± 0.03 | OG | Ser45 | (0.17) |
| 3.46 ± 0.07 | ND2 | Asn128 | (1.15) | |
| O2 | 2.93 ± 0.04 | NZ | Lys165 | (−0.70) |
| 3.23 ± 0.12 | ND2 | Asn195 | −5.20 | |
| 3.12 ± 0.07 | ND2 | Asn128 | (1.15) | |
a
Average B factors among four chains (chain A–D) of the main chain in the FMN‐bound form are subtracted by those of the FMN‐free form. The values exhibiting unremarkable changes that are marked by black symbol in Fig. 6A are shown in parentheses.