Table 1.
TP53 mutation in AGS cells related to CHAC1 overexpression
| Total mutated clones | Mutations causing amino acid substitutions | P value* | |
|---|---|---|---|
| AGS cells with CHAC1 overexpression by transfection | |||
| Day 4 CHAC1‐WT | 5/60 | 2/70 740 (0.28/104) | N.S. |
| Day 8 CHAC1‐WT | 10/59 | 7/69 561 (1.01/104) | 0.0074 |
| Day 16 CHAC1‐WT | 20/59 | 13/69 561 (1.87/104) | 0.0001 |
| Day 16 CHAC1‐MT | 0/50 | 0/58 950 (0.00/104) | N.S. |
| Day 16 control | 0/60 | 0/70 740 (0.00/104) | – |
| AGS cells with CHAC1 overexpression induced by H. pylori infection | |||
| Uninfected | 0/60 | 0/70 740 (0.00/104) | – |
| cagA (−) H. pylori | 0/59 | 0/69 561 (0.00/104) | N.S. |
| cagA (+) H. pylori | 14/59 | 8/69 561 (1.15/104) | 0.0037 |
| cagA (+) H. pylori + scrambled siRNA | 10/53 | 6/62 487 (0.96/104) | 0.0107 |
| cagA (+) H. pylori + CHAC1 siRNA | 0/58 | 0/68 382 (0.00/104) | N.S. |
*P values for the number of mutated bases causing amino acid substitutions were evaluated by Fisher's exact test.
Total mutated clones indicates the total number of mutations per total number of clones examined; Mutations causing amino acid substitutions indicates the number of mutated bases causing amino acid substitutions per total number of base pairs sequenced. Frequency of mutations causing amino acid substitutions per 104 base pairs is shown in parentheses. AGS cells were subjected to H. pylori infection for 16 days (see Methods). N.S., not significant.