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. 2018 Feb 19;37(7):e98499. doi: 10.15252/embj.201798499

Figure 5. Pno1 interacts with the rRNA of the platform.

Figure 5

  1. Overview of the pre‐40S ribosome. The rRNA is represented as spheres (gray) and the r‐proteins as surfaces (light blue). In the pre‐40S particle, helix 28 (cartoon in pink) is highly distorted compared to the mature conformation (dark blue, PDB 4V88; Ben‐Shem et al, 2011) in which it would clash with Pno1 (green).
  2. Pno1 interacts with 20S rRNA nucleotides 1787–1800. Nucleotide A1794 is specifically recognized by the C‐terminal KH domain of Pno1, which contains a conserved GxxG RNA binding motif (blue). A1794 is flipped out, which twists the RNA such that the bases that follow bind to the surface of Pno1 (Fig EV4D).
  3. Surface representation of Pno1 colored by residue conservation. Highly conserved residues (red) form the surface interacting with the 20S rRNA (Appendix Fig S4).