Figure 4.
Comparison of anti‐HCMV activity and cytotoxicity between flavopiridol and tricin. HEL cells (1.5 × 105 cells) plated in 24‐well plates were infected with HCMV and treated with various concentrations of flavopiridol or tricin during and after infection for 7 days. The antiviral activity was then determined in a PFU reduction assay. Cytotoxicity of the two compounds was determined by incubating HEL cells in 96‐well plates with various concentrations of flavopiridol or tricin for 7 days in triplicate, followed by measurement of LDH release as described in the Materials and methods.