Figure 2.
Maximum DDAVP-stimulated receptor activities. HEK293 cells expressing cAMP-inducible luciferase, Renilla luciferase and WT or L57R V2R were treated with 10−7 M DDAVP with and without 10−6 M SR121463. Luciferase activity, a measure of intracellular cAMP generation, is normalized to Renilla luciferase activity and expressed as percentage of maximum WT V2R activity. Data are mean +/− SEM of 2 experiments, each performed in triplicate. NS = not significant; * = P < 0.05; *** = P < 0.001.