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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Mar 16;498(4):1022–1027. doi: 10.1016/j.bbrc.2018.03.108

Figure 2. Time course of in vitro processing assay of hairpin RNAs by Dicer-2 ± Loqs-PD.

Figure 2

Hairpin RNAs (100 nM, uniformly 32P-radiolabeled) with (1) a 5′ monophosphorylated blunt end, (2) a 5′ hydroxyl blunt end, (3) a 5′ monophosphorylated, 3′ 2-nt overhang end, or (4) a 5′ hydroxyl, 3′ 2-nt overhang end were incubated with 8 nM Dicer-2 ± Loqs-PD in the presence or absence of 1 mM ATP and/or 25 mM inorganic phosphate. Data are mean ± s.d. for three independent trials.