Figure EV1. Analysis of sDR6 level in WT primary neurons upon MT1‐MMP knockdown.

Specific short hairpin RNAs targeting MT1‐MMP (sh1 and sh2) were expressed in primary neurons using a lentiviral system. A scrambled shRNA sequence was used as negative control. Levels of full‐length DR6 (DR6 FL) and its released ectodomain (sDR6) were analyzed in cell lysates and culture supernatant, respectively (n = 6 for each group), showing that upon MT1‐MMP knockdown, full‐length protein and its processing were not affected. Western blot quantifications are represented using box plots showing the first and the third quartile together with the median. The whiskers show the maximum and the minimum data point. Statistical test: unpaired Student's t‐test with two‐tailed distribution. Source data are available online for this figure.