Figure EV5. Rescue of knockout phenotype by sDR6.

In order to test whether sDR6 is able to rescue the myelination phenotype, WT and DR6 KO cultures were incubated with complete medium supplemented with 1 μg/ml murine recombinant sDR6. Quantification of myelin segments (seg., MBP‐positive, green) showed that the treatment with sDR6 was sufficient to downregulate myelination. Neurons were labeled with β3‐tubulin (red). Myelin segment quantification is represented using box plots showing the first and the third quartile together with the median. The whiskers show the maximum and the minimum data point. n = 6 for each group; scale bar = 500 μm.