Figure EV7. Lysosomal activity is required to re‐activate mTORC1 following HD tamoxifen injury.

1. At peak metaplasia stages in Gnptab ^−/+ tissue, pS6 is re‐expressed throughout the stomach epithelium, including intense staining within the pit and metaplastic base. Scale bars: 50 μm; pullout, 25 μm.
2. In Gnptab ^−/− tissue, pS6 is not reactivated in the base, indicating lysosomal activity is required for mTORC1 re‐activation at later stages following injury. Boxed regions are shown at higher magnification at right with a representative base (in which pS6 remains inactive without lysosomal activity) outlined by dotted line. Lysosomal activity appears dispensable for pit cells (at top of gastric unit) mTORC1 activity. Scale bars: 50 μm; pullout, 25 μm.