Fig. 1.

Binding of AR20.5 Fab to a MUC1 peptide and glycopeptide. Binding affinity of AR20.5 Fab fragment to synthetic MUC1 peptide (APDTRPAP) (dashed line, triangles) and glycopeptide (APDTnRPAP) (solid line, closed circles) was determined by monitoring the ligand-binding induced change in the fluorescence of labeled AR20.5. Error bars represent the standard error of the mean from three separate experiments. MUC1 glycopeptide binds to AR20.5 with ~20-fold higher affinity compared to the peptide.