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The activation of the p53-p21-retinoblastoma protein (Rb) pathway induced by cyclic mechanical tension (CMT) with 20% elongation. (A and B) The quantitative PCR analysis and western blot analysis of p53, p16, p21 and Rb in nucleus pulposus (NP) cells subjected to CMT with 20% elongation for 24 h. *P-value <0.05 error bars represent standard error of the mean (SEM). Control, NP cells that were cultured under the same conditions and were kept static; 20%, CMT with 20% elongation.
