Table I.
Antibody | Conjugation | Manufacturer | Dilution (FACS) | Dilution (IF) | Dilution (IHC) | Dilution (WB) |
---|---|---|---|---|---|---|
Sca-1 | FITC | Biolegend | 1:100 | |||
CD34 | APC | Biolegend | 1:100 | |||
CD44 | PE | Biolegend | 1:100 | |||
CD45 | Percp | Biolegend | 1:100 | |||
Nestin | Abcam | 1:100 | 1:200 | 1:1,000 | ||
DCX | Abcam | 1:100 | 1:200 | 1:500 | ||
NF-H | Abcam | 1:200 | 1:200 | 1:1,000 | ||
Goat anti-rabbit | Rhodamine | Zhongshan | 1:100 | |||
Goat anti-mouse | Rhodamine | Zhongshan | 1:100 | |||
Goat anti-rabbit | HRP | Zhongshan | 1:400 | |||
Goat anti-mouse | HRP | Zhongshan | 1:400 | |||
Goat anti-rabbit | HRP | Zhongshan | 1:5,000 | |||
Goat anti-mouse | HRP | Zhongshan | 1:5,000 |
FACS, fluorescence-activated cell sorting; NF-H, neuronal specific filament; HRP, horseradish peroxidase; DCX, doublecortin.