Skip to main content
. 2018 Feb 28;41(6):3185–3194. doi: 10.3892/ijmm.2018.3517

Table I.

The antibodies.

Antibody Conjugation Manufacturer Dilution (FACS) Dilution (IF) Dilution (IHC) Dilution (WB)
Sca-1 FITC Biolegend 1:100
CD34 APC Biolegend 1:100
CD44 PE Biolegend 1:100
CD45 Percp Biolegend 1:100
Nestin Abcam 1:100 1:200 1:1,000
DCX Abcam 1:100 1:200 1:500
NF-H Abcam 1:200 1:200 1:1,000
Goat anti-rabbit Rhodamine Zhongshan 1:100
Goat anti-mouse Rhodamine Zhongshan 1:100
Goat anti-rabbit HRP Zhongshan 1:400
Goat anti-mouse HRP Zhongshan 1:400
Goat anti-rabbit HRP Zhongshan 1:5,000
Goat anti-mouse HRP Zhongshan 1:5,000

FACS, fluorescence-activated cell sorting; NF-H, neuronal specific filament; HRP, horseradish peroxidase; DCX, doublecortin.