Table II.
Primers | Sequences | Product size | Manufacturer |
Nestin | Forward: 5′-CTGGGCAAGTGGAACGTAGA-3′ | ||
Reverse: 5′-CCTCCCACCGCTGTTGAT-3′ | 150 bp | Invitrogen | |
DCX | Forward: 5′-CCTCAGGGAGTGCGCTACAT-3′ | ||
Reverse: 5′-CGACCAGTTGGGATTGACATT-3′ | 150 bp | Invitrogen | |
NF-H | Forward: 5′-GATGGCCCTGGATATTGAGA-3′ | ||
Reverse: 5′-TTCGCTTTTGACTTTTATGTGAG-3′ | 148 bp | Invitrogen | |
β-actin | Forward: 5′-TGTCACCAACTGGGACGATA-3′ | ||
Reverse: 5′-GGGGTGTTGAAGGTCTCAAA-3′ | 165 bp | Sangon Biotech |
NF-H, neuronal specific filament; DCX, doublecortin.