Table I.
Primer sequences used in semi-quantitative RT-PCR analysis.
| ASG (Exon) | Forward primer | Reverse primer |
|---|---|---|
| ADAMTS9 (Exon 31) | CTACAGGCAAAGGCTGGTCTCC | CAGGACAGTCCCTCAGGTAGCA |
| FNDC1 (Exon 11) | GCAATCGGTCTTCGCTGAGGAG | GCATCGGTGTGGTGGAGGAGTA |
| GDF15 (Exon 2) | GCTGCTGTCACCTGGAGACTGT | GCCGCTGTCTGTCCTGTGCATA |
| KRCC1 (Exon 3) | GAGTTTGATTCCAGGCCCAGGT | CTGCCTTTCTCTGCCCTCCTCT |
| NPTN (Exon 2) | GGGTTTGTCAAGTCGCCCATGT | TCAGCACACTCACGCCGTTTG |
| OGN (Exon 8) | TGGTTGTAGCAGACCGCCATCT | GGGAAGGTCACTGGGAGCACTT |
| PREB (Exon 4) | TGGTTACTGTGGGCTGGGACTT | CCTGGTAGCGGTACGGTGTGTT |
| SEC31A (Exon 23) | ACCAGCCAGCCCAGCAGTATT | TTCCAGGAGGCAGTGCATAAGC |
| TJP1 (Exon 27) | GCAGAAGCCTCATCTCCAGTCC | GCGACGGCAATGACACTCCTT |
| GAPDH | GTCCATGCCATCACTGCCACTC | GATGACCTTGCCCACAGCCTTG |
ASG, alternative splicing gene; ADAMTS9, a disintegrin and metalloproteinase with thrombospondin motifs 5; FNDC1, fibronectin type III domain containing 1; GDF15, growth and differentiation factor 15; KRCC1, lysine-rich coiled-coil 1; NPTN, neuroplastin; OGN, osteoglycin; PREB, prolactin regulatory element binding; SEC31A, SEC31 homolog A, COPII coat complex component; TJP1, tight junction protein 1.