Table I.
Sequences of polymerase chain reaction primers used in the present study.
| Gene name | Primer | Primer sequence (5′-3′) | Product length (bp) |
|---|---|---|---|
| Ig Vγ | External sense | VH1:GAGGTGCAGCTCGAGGAGTCTGGG | |
| VH2:CAGGTGCAGCTCGAGCAGTCTGGG | |||
| VH3:CAGGTACAGCTCGAGCAGTCAGG | |||
| VH4:CAGGTGCAGCTGCTCGAGTCGGG | 280–300 | ||
| External antisense | CH1:ACACCGTCACCGGTTCGG | ||
| Internal antisense | FR2:TGGRTCCGVCAGSCYCCNGG | ||
| Internal antisense | JH:AACTGCAGAGGAGACGGTGACC | ||
| Ig Vκ | External sense | GACATCGAGCTCACCCAGTCTCC | 340–360 |
| External antisense | CGGGAAGATGAAGACAGATGGTGC | ||
| Internal sense | GAAATTGAGCTCACGCAGTCTCCA | ||
| Internal antisense | TGGTGCAGCCACAGTTCGTT | ||
| Ig Cγ | Sense | AGGACTCTACTCCCTCAGCAG | 566 |
| Antisense | TCAGGCTGACCTGGTTCTTG | ||
| Ig Cκ | Sense | TGAGCAAAGCAGACTACGAGA | 231 |
| Antisense | GGGGTGAGGTGAAAGATGAG | ||
| Ig Cλ | Sense | GGGACCAAGCTCACCGTCCTAG | 316 |
| Antisense | TCTTCTCCACGGTGCTCCCTTC | ||
| Ig Cγ1 | Sense | GGGCTTCCAAGCCAACAGGGCAGGACA | 603 |
| Antisense | GTTTTGTCACAAGATTTGGGCTC | ||
| Ig Cγ2 | Sense | GGGCTTCCAAGCCAACAGGGCAGGACA | 597 |
| Antisense | GTGGGCACTCGACACAACATTTGCG | ||
| Ig Cγ3 | Sense | AGGTGGGCAGGCTTCAGGCACCGAT | 670 |
| Antisense | TTGTGTCACCAAGTGGGGTTTTGAGC | ||
| Ig Cγ4 | Sense | GATGGCGTGGAGGTGCATAA | 212 |
| Antisense | GTGTACACCTGTGGCTCTCG | ||
| AID | Sense | GAGGCAAGAAGACACTCTG | 647 |
| Antisense | GTGACATTCCTGGAAGTTGC | ||
| RAG1 | External sense | TGGATCTTTACCTGAAGATG | 327 |
| External antisense | CTTGGCTTTCCAGAGAGTCC | ||
| Internal sense | CACAGCGTTTTGCTGAGCTC | ||
| Internal antisense | AGCTTGCCTCAGGGTTCATG | ||
| RAG2 | External sense | TGGAAGCAACATGGGAAATG | 193 |
| External antisense | CATCATCTTCATTATAGGTGTC | ||
| Internal sense | TTCTTGGCATACCAGGAGAC | ||
| Internal antisense | CTATTTGCTTCTGCACTG | ||
| CD19 | Sense | AAGGGGCCTAAGTCATTGCT | 347 |
| Antisense | CACGTTCCCGTACTGGTTCT | ||
| β-actin | Sense | AGAGCTATGAGCTGCCTGAC | 121 |
| Antisense | AATTGAATGTAGTTTCATGGATG |
AID, activation-induced cytidine deaminase; C, constant; CD19, cluster of differentiation 19; Ig, immunoglobulin; RAG, recombination activating gene; V, variable.