Figure 3.

Bacterial dysbiosis is pre-existing at the time of IPAA surgery. 16S rRNA gene sequencing was performed on tissue samples to analyze the mucosa-associated bacteria at the time of IPAA surgery. Relative abundance was calculated and compared between siblings A (red) and B (blue). Patient B subsequently developed pouchitis while patient A did not. (A) Mucosa-associated bacteria from rectal tissue at the time of IPAA. (B) Mucosa-associated bacteria from terminal ileum tissue at the time of IPAA.