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. 2017 Jan 4;11(6):737–750. doi: 10.1093/ecco-jcc/jjw219

Figure 7.

Figure 7.

Inhibition of NLRP3 inflammasome with glyburide before disease onset markedly suppressed multiple major proinflammatory cytokines/chemokines and effectively prevented or delayed spontaneous colitis. IL-10-/- mice [1 month old] were intraperitoneally administrated with glyburide every other day for 2.5 months [the final age of mice: 3.5 months]. [A] Colon and stool morphology of wild-type [WT] and IL-10-/- mice at 1 month [a] and 3.5 months old [b]; percentage of macroscopic colitis [c]. [B] Haematoxylin and eosin [H&E] staining and histological scores of colonic sections of IL-10-/- mice at 1 month old [top two panels], and 3.5 months old [bottom two panels] with or without glyburide treatment. Leukocyte infiltration [blue arrows]; loss of crypt and goblet cells [red arrows]. [C] Expression of multiple proinflammatory cytokines and chemokines, as measured by multiplex enzyme-linked immunosorbent assay [ELISA] [see Supplementary Methods, available at ECCO-JCC online]; *p < 0.05 [glyburide-treated vs controls].