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. 2016 Dec 15;11(6):751–760. doi: 10.1093/ecco-jcc/jjw212

Figure 6.

Figure 6.

Dextran sodium sulphate DSS induction of receptor activator of nuclear factor kappa-B ligand RANKL in PP and colon is reduced by LTβR blockade. a. Immunostaining of Peyer’s patches [PP] and colonic infiltrates for RANKL [orange] show expression in stromal cells, with apparent decreased signal in DSS/sLTβR-treated mice, though no apparent effect on M cells [red]. Scale bar: 40 µm. b. Although colonic M cell induction was not affected by LTβR blockade [Figure 5e], morphometric analysis of RANKL:M cell ratios shows alteration of ratios indicating decrease in RANKL signal relative to M cell signal in both Peyer’s patches and colonic infiltrates. Each data point reflects a calculated RANKL:M cell ratio from a single microscopy image; multiple images were acquired from each of two different mice per experimental condition. Note that the y-axis is split, in order to show more clearly that among the LTβR blockade group, many of the RANKL:M cell ratios were zero [***P < 0.0005; ****P < 0.0001].