Figure 3.

NTPDase2-deficient mice develop exacerbated inflammation in dextran sodium sulphate [DSS]-induced colitis. A. Daily clinical disease activity index in DSS induced colitis. This index was calculated from individual scores for weight loss, bleeding and stool consistency as described in Supplementary Table 1, available as Supplementary data at ECCO-JCC online. DSS-induced colitis is most severe in Entpd2 null mice when compared with wild type and Entpd3 null mice [n = 5 mice per group, this figure represents one out of three independent experiments that showed the same trends]. B. Haematocrit was measured after 7 days of treatment with 3% DSS. C. Representative haematoxylin and eosin [H&E]-stained tissue sections of mouse colon after 7 days of treatment with 3% DSS. Scale bar represents 200 µm. D, E. Histology scores of colitis using Kim’s [D] and Wirtz’s [E] methods. Error bars show standard deviations in all panels; p-values are calculated using one-way analysis of variance [ANOVA] referencing to the wild type group [*p < 0.05, **p < 0.005]. Area under the curve [AUC] of panel A was used for comparison.