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. 2017 Aug 18;12(2):217–229. doi: 10.1093/ecco-jcc/jjx115

Figure 3.

Figure 3.

NPs-PEG-FA are taken up by macrophages and epithelial cells through folate receptor-mediated endocytosis. A: Colon-26 cells and activated Raw 264.7 cells were incubated with NPs-PEG/DiL or NP-PEG-FA/DiL for 6 h. Here, DiL was a fluorescent dye and used as a tracker. The colours are as follows: blue channel, DAPI; green channel, FITC; and red channel, DiL. Panels: a, colon-26 cells incubated with NPs-PEG/DiL; b, colon-26 cells incubated with NPs-PEG-FA/DiL; c, activated Raw 264.7 cells incubated with NPs-PEG/DiL; and d, activated Raw 264.7 cells incubated with NPs-PEG-FA/DiL. B and C: Flow cytometry was used to quantify the fluorescence intensity of DiL in colon-26 cells [B] and activated Raw 264.7 cells [C] treated as described above and incubated for 6 h. In all experiments, n = 5; the scale bar in [A] = 20 µm.