Table II.
Catalytic parameters of Bm-LS variants from library S173/S422
| Enzyme | KM (mM) | kcat (s−1) | kcat/KM (s−1 mM−1) | ΔΔG# (kcal mol−1) |
|---|---|---|---|---|
| S173S/S422S (WT) | 10.8 ± 1.5 | 197.9 ± 3.3 | 18.33 | 0 |
| S173S/S422A | 19.9 ± 2.4 | 97.9 ± 4 | 4.91 | 0.8 |
| S173A/S422S | 3.9 ± 0.3 | 3.8 ± 0.1 | 0.97 | 1.8 |
| S173A/S422A | 7.9 ± 0.9 | 2.8 ± 0.1 | 0.35 | 2.4 |
| S173A/S422G | 23.7 ± 2.9 | 5.9 ± 0.1 | 0.24 | – |
| S173A/S422T | 43.1 ± 3.7 | 17.8 ± 0.4 | 0.41 | – |
| S173G/S422S | 27.7 ± 5.5 | 8.7 ± 0.4 | 0.31 | 2.48 |
| S173G/S422G | 50.1 ± 7.2 | 28.3 ± 1.3 | 0.56 | 2.14 |
| S173G/S422A | 52.6 ± 5.3 | 45.8 ± 1.6 | 0.87 | – |
| S173T/S422S | 41.8 ± 7.0 | 107.9 ± 4.2 | 2.58 | – |
| S173T/S422T | 82.2 ± 9.4 | 30.3 ± 1.8 | 0.36 | – |