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Catalytic parameters of Bm-LS variants at positions 421 and 439
| Enzyme | KM (mM) | kcat (s−1) | kcat/KM(s−1 mM−1) | ΔΔG# (kcal mol−1) |
|---|---|---|---|---|
| Wild-type | 10.8 ± 1.5 | 197.9 ± 4 | 18.32 | – |
| Y421A | 421.0 ± 42.3 | 13.8 ± 1.3 | 3.27 × 10−2 | 3.96 |
| Y421M | 214.0 ± 14.5 | 13.0 ± 0.9 | 6.07 × 10−2 | |
| Y421F | 31.7 ± 2.8 | 17.5 ± 1.6 | 0.55 | 2.15 |
| Y421W | 106.1 ± 15.7 | 18.9 ± 2.8 | 0.18 | |
| Y439A | 621.7 ± 66.2 | 5.4 ± 0.6 | 0.86 × 10−2 | 4.71 |
| Y439M | 441.9 ± 38.6 | 61.0 ± 1.9 | 0.14 | |
| Y439F | 92.9 ± 19.6 | 183.6 ± 7.6 | 1.98 | 1.36 |
| Y439W | 368.2 ± 33.3 | 167.4 ± 4.5 | 0.45 |
