Table IV.
Hydrolysis and transfructosylation values for Bm-LS on sucrose
| Enzyme | Transfructosylation (%) | Hydrolysis (%) |
|---|---|---|
| Library S173/S422 | ||
| WT | 11.0 | 89.0 |
| S173S/S422A | 15.6 | 84.4 |
| S173A/S422S | 32.3 | 67.7 |
| S173A/S422A | 25.2 | 74.8 |
| S173A/S422G | 18.6 | 81.4 |
| S173A/S422T | 25.8 | 74.2 |
| S173G/S422S | 16.8 | 83.2 |
| S173G/S422G | 20.6 | 79.4 |
| S173G/S422A | 14.7 | 85.3 |
| S173T/S422S | 9.4 | 90.6 |
| S173T/S422T | 20.3 | 79.7 |
| Variants at other positions | ||
| Y421A | 8.1 | 91.9 |
| Y421M | 12.1 | 87.9 |
| Y421F | 23.3 | 76.7 |
| Y421W | 9.3 | 90.7 |
| Y439A | 8.1 | 91.9 |
| Y439M | 9.8 | 90.2 |
| Y439F | 8.7 | 91.3 |
| Y439W | 8.3 | 91.7 |