Effects of TP on the phosphorylation of IκBα, NF-κB and ERK1/2, and the expression of iNOS, COX2, caspase-3, Bcl2, Bax and LOX-1, as determined by western blot analysis. (A) Relative expression levels of IκBα, NF-κB, ERK1/2, iNOS, COX2, caspase-3, Bcl2, Bax and LOX-1 were determined. (B) Semi-quantification of western blot analysis was used to measure the phosphorylation of IκBα, NF-κB and ERK1/2. (C) Expression levels of iNOS, COX2, caspase-3, Bcl2, Bax and LOX-1 were analyzed by semi-quantification of western blot analysis. ***P<0.001, compared with the control group. #P<0.05, ###P<0.001, compared with the I/R group (n=3). Bax, Bcl2-associated X protein; Bcl2, B-cell lymphoma 2; COX2, cyclooxygenase 2; ERK1/2, extracellular signal-regulated kinase 1/2; IκBα, NF-κB inhibitor α; iNOS, inducible nitric oxide synthase; I/R, ischemia/reperfusion; LOX-1, lectin-like oxidized low-density lipoprotein receptor-1; NF-κB; nuclear factor-κB; p-, phosphorylated; TP, triptolide.