Figure 5.

ST1926-ameliorates inflammation response in mice induced by lipopolysaccharide (LPS) is dependent on nuclear factor-κB (NF-κB) signaling pathway. Immnohistochemical analysis of pro-inflammatory cytokines of (A) tumor necrosis factor-α (TNF-α) and (B) interleukin-1β (IL-1β) were assessed. (C) RT-qPCR assays were conducted to explore mRNA levels of TNF-α, IL-1β, IL-18 and IL-6. (D) Western blot assay was performed to investigate NF-κB signaling pathway, including signals of inhibitor-κB kinase (IKKα), inhibitor-κB kinase-α (IκBα) and NF-κB phosphorylation, and the quantified levels are displayed. The data are presented as mean ± SD (n=8). ^*p<0.05 and ^**p<0.001 vs. the control (Con); ⁺p<0.05, ⁺⁺p<0.01 and ⁺⁺⁺p<0.001 vs. LPS-induced mice (LPS).