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. 2018 Mar 7;41(6):3115–3126. doi: 10.3892/ijmm.2018.3541

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Copyright: © Cao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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SUMOylation exerts an impact on the protein level and localization of Aurora B in granulosa cells. (A) granulosa cells were co-transfected with HA-Aurora B (2 μg) and Flag-SUMO2 (2, 3, 4, and 5 μg), and the protein level of Aurora B was determined by western blotting. (B) Scanning intensity analysis of the western blots. Each experiment was repeated three times, and the values are presented as the mean ± standard deviation. ^**P<0.01 cf. the 2 μM value. (C) The co-transfection of HA-Aurora B or HA-Aurora B^K207R with Flag-SUMO2. Localization was observed using immunocytochemistry. FITC indicated the localization of HA-Aurora B and/or HA-Aurora B^K207R, Cy3 indicated the localization of Flag-SUMO2, and DAPI indicated the granulosa cells' nuclei (the images are magnified at ×20). SUMO2, small ubiquitin-related modifier 2; FITC, fluorescein isothiocyanate.