Fig. 8.

Immunological challenge in rats with HEP-DiPalm and ELISA assessment. A set of three rats (#1, 2, 3) was boosted with a series of HEP-conjugates in a long-term study (see Supplementary Fig. 4) then tested for the induction of an IgM or IgG response by ELISA (representative set of assays with averaged triplicate wells with standard deviation is presented). Overall, the signal from sera of rats before and after injection with HEP-DiPalm micelles (prelipid, white; postlipid, black) tested in the control wells coated with bovine serum albumin (BSA) was equivalent to the wells coated with HEP-BSA (HEP) or HEP-DiPalm (DiPalm) indicating that HEP is not significantly immunogenic, therefore, should be useful in multidose or long-term therapeutics.