Figure 5.

Pigment epithelial-derived factor (PEDF)-induced hypoxic primary cardiomyocyte mitophagy through FUNDC1 is dependent on ULK1 signaling pathway. Primary cardiomyocytes were maintained in normoxic or hypoxic conditions for 4 h with or without PEDF (10 nM). RNA interference assays were used to silence PEDF-R. PKC-α inhibitor Go6976 (1 µM) was added. ULK1, FUNDC1, p-FUNDC1 and LC3-II levels were tested by western blotting (n=4, ^*p<0.05). Data were expressed in fold induction, relative to control. Values are means ± SD.