Fig. 5.

IgG binding to FcRL5 is influenced by IgG glycan compositions. (A) Un-manipulated, neuraminidase-treated (desialylated) and neuraminidase- and galactosidase-treated (degalactosylated) IgG pools (IgG1 and IVIG) were slot blotted and the impact of differential glycosylation assessed by detection with FcRL5 (left, IVIG shown). Band intensities were quantified for both polyclonal IgG1 and IVIG at three different antibody concentrations (right). (B) As a test of equivalent antibody loading, the same membrane was stripped and re-blotted to detect (left, IVIG shown) and quantify (right) total IgG via detection with an anti-human IgG secondary. This figure is available in black and white in print and in color at Glycobiology online.