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. 2018 Mar 9;41(6):3243–3252. doi: 10.3892/ijmm.2018.3552

Figure 1.

Figure 1

PEDF reduces myocardial infarct size and improves cardiac function under MI/R conditions. (A) Representative figures of the Evans Blue/TTC-stained myocardial tissues in each indicated experimental condition, with (B) quantification of the infarct size (n=6). (C) Representative images of TUNEL staining of cardiomyocyte apoptosis (white arrow), with (D) quantification. Cardiomyocyte apoptosis was measured by TUNEL staining; cardiomyocytes were identified using α-sarcomeric actin antibodies. TUNEL staining for cardiomyocyte apoptosis (red), DAPI for nuclear staining (blue) and α-sarcomeric actin for cardiomyocytes (green) in the border zone of the infarcted left ventricle from all experimental groups (scale bar=20 μm; n=6). (E) Left ventricular ejection fraction prior to dobutamine injection determination by echocardiography (n=6). (F) Left ventricular fractional shortening prior to dobutamine injection determination by echocardiography (n=6). (G) Δ left ventricular ejection fraction following dobutamine injection by echocardiography (n=6). (H) Δ left ventricular fractional shortening following dobutamine injection by echocardiography (n=6). Data are expressed as the mean ± standard error of the mean. *P<0.05, with comparisons indicated by lines. PEDF, pigment epithelium-derived factor; MI/R, myocardial ischemia/reperfusion; TTC, 2,3,5-triphenyltetrazolium; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.