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. 2018 Mar 9;41(6):3243–3252. doi: 10.3892/ijmm.2018.3552

Figure 4.

Figure 4

PEDF decreases H/R-induced apoptosis via PEDF-R in cultured H9c2 cells. H9c2 cells were maintained in normoxic or H/R conditions for 8/2 h with or without PEDF (10 nM). RNA interference assays were used to silence PEDF-R. (A) Samples were collected for western blotting to analyze the expression of cleaved casp3 protein, with (B) quantification performed using ImageJ software (n=4). (C) Effect of PEDF on H9c2 cells apoptosis, with (D) quantification. TUNEL (red) staining was performed for each group. Nuclei were stained with DAPI (blue). Cells that were TUNEL and DAPI-positive were apoptotic (indicated by the arrows), while DAPI positive were control cells (scale bar=50 μm; n=4). Data are expressed as the mean ± standard error of the mean. *P<0.05, with comparisons indicated by lines. PEDF, pigment epithelium-derived factor; H/R, hypoxia/reoxygenation; PEDF-R, pigment epithelium-derived factor receptor; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; si, small interfering.