Table II.

Antibody list.

Primary antibody	Company	Species	Concentration	Secondary antibody	Company	Species	Concentration	Cat. nos.
IGF	ZSGB-BIOa	Rabbit	1:50	Cy3	Jacksonb	Goat anti-rabbit	1:200	111-165-003
BDNF	ZSGB-BIO	Rabbit	1:50	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
NGF	ZSGB-BIO	Rabbit	1:50	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
GDNF	ZSGB-BIO	Rabbit	1:100	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
TGF-β1	Abcamc	Rabbit	1:100	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
CNTF	Biossd	Rabbit	1:50	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
GFAP	ZSGB-BIO	Rabbit	1:50	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003
NeuNe	Bioss	Rabbit	1:100	Cy3	Jackson	Goat anti-mouse	1:200	111-165-003
NeuNf	Bioss	Rabbit	1:100	488	Invitrogeng	Goat anti-rabbit	1:100	A-11034
Nestin	ZSGB-BIO	Rabbit	1:50	Cy3	Jackson	Goat anti-rabbit	1:200	111-165-003

^aZSGB-BIO; Origene Technologies, Inc., Beijing, China;

^bJackson,;

^cAbcam, Cambridge, MA, USA;

^dBioss, Woburn, MA, USA;

^eNeuN detection in vitro;

^fNeuN detection in vivo;

^gInvitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA. Cell identification, Nestin. Differentiation identification: GFAP and NeuN. NTF: IGF, BDNF, NT-3, GDNF, TGF-β1 and CNTF. NeuN, neuron-specific nuclear protein; IGF, insulin-like growth factor; BDNF, brain-derived neurotrophic factor; NGF, nerve growth factor; GDNF, glial cell line-derived neurotrophic factor; TGF-β1, transforming growth factor-β1, CNTF, ciliary neurotrophic factor; GFAP, glial fibrillary acidic protein.