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. 2018 Jan 25;243(5):473–480. doi: 10.1177/1535370218754848

Figure 2.

Figure 2.

Ectopic expression of miR-448 suppresses proliferation, colony formation, migration, and invasion in breast cancer cells in vitro. (a) miR-448 levels in MDA-MB-231 and MCF-7 cells transfected with scramble miRNA, miR-448-mimics, or miR-448-inhibitor. (b and c) qRT-PCR analysis of relative TWIST1 expression in these breast cancer cells after treating with miR-448 mimics or inhibitors. (d) MTT assay of MDA-MB-231 and MCF-7 cells transfected with scramble miRNA, miR-448-mimics, or miR-448-inhibitor. (e and f) Representative images and quantification of the colony formation assay. (g and i) Migration and invasion assay of MDA-MB-231 and MCF-7 cells transfected with scramble miRNA, miR-448-mimics, or miR-448-inhibitor. (h and j) The cell number of migrated cells were counted in randomly selected fields and presented in bar graph (means ± SD; *P < 0.05, Student’s t test). (A color version of this figure is available in the online journal.)