Figure 1.

Generation, growth, and survival of Fryl^−/− mice. (a) Schematic presentation of truncated Fryl gene by pU-21T gene trap vector. Arrows indicate approximate positions for genotyping primers. Genotyping PCR primer sites for wild type (gray arrows) and mutant alleles (black arrows) were indicated. Exons were shown as black boxes with numbers. (b) Fryl mutant mouse genotyped based on PCR (201 and 247 base pairs for wild and mutant alleles, respectively). M: size marker. (c) Western blot analysis showing no Fryl expression in Fryl^−/− mouse. GAPDH was used as internal control. +/+: wild type; +/−: heterozygous; −/−: homozygous. Body weights of Fryl^−/− mice were plotted according to gender and genotypes. (d) Body weights of female Fryl^+/+ (n = 4), Fryl^+/− (n = 4) and Fryl^−/− (n = 6) mice were plotted. (e) Body weights of male Fryl^+/+ (n = 4), Fryl^+/− (n = 4), and Fryl^−/− (n = 3) mice were plotted. (f) Among them, one female and one male Fryl^−/− mice died at seven and six weeks of age, respectively, as shown in the plot for the number of Fryl^−/− survivors. Values are means ± SD. *P < 0.05. (A color version of this figure is available in the online journal.)