Figure 5.

Kidneys of Fryl^−/− newborns and embryos. Kidney sections from wild (a) and Fryl^−/− (b) live neonates were stained with hematoxylin and eosin. Detachment of tubular wall cell layer and materials filling the space were observed in live Fryl^−/− neonates. Wild (c) and Fryl^−/− (d) embryos aged 18.5 days showed normal appearance. However, almost all cells in kidneys from Fryl^−/− embryos (f) had picnotic nuclei apart from their wild littermates (e). Cells with picnotic nuclei were apoptotic cells as demonstrated by TUNEL assay for wild (g) and Fryl^−/− (h) embryos. Upper right inlets in G and H show magnified photos of small squares. Counterstain; methyl green. Magnification: 400× for (a), (b), (e), (f), (g), and (h). (A color version of this figure is available in the online journal.)