Figure 2.

Indices for immunological and renal function. (a) Spleen weight and serum albumin/globulin (A/G) ratio. (b) Blood urea nitrogen (BUN) and serum creatinine (Cr). Box-and-whisker plot. The Kruskal–Wallis test was used to compare data among groups. Multiple comparisons were performed using Dunnett's test to compare the parameters with the healthy control (Cont) group. For the comparison of groups with pyometra (Pyo+), those without pyometra (Pyo−), and ovariectomy (OVX), Scheffé's method was used. P⁻, P⁺, and O denote the significant difference with Pyo−, Pyo+, and OVX groups, respectively (P < 0.05). P⁻P⁻, P⁺P⁺, and OO denote the highly significant difference with Pyo−, Pyo+, and OVX groups, respectively (P < 0.01).