Table 1. Primers and the probe used to amplify the full-length and quantitative PCV1.
Name | Direction | Sequence | Position |
---|---|---|---|
PCV1-full length | Forward | GCATGGATCCATCACTTCGTAATGGT | 1010–1035 |
Reverse | GCATGGATCCAAAAAAGACTCAGTAA | 1035–1010 | |
PCV1-RTPCR | Forward | TGGCCCGCAGTATTTTGATT | 785–804 |
Reverse | CAGCTGGGACAGCAGTTGAG | 856–838 | |
Probe | FAM-CCAGCAATCAGGCCCCCCAGGAAT-TAMRA | 806–829 |