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. 2017 Aug 31;163(9):1292–1305. doi: 10.1099/mic.0.000515

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Fig. 3. — Effect of hybrid genes on growth and phenotype. (a) Effect of hybrid constructs on the growth of L. lactis. X, FI5876; ●, FI5876ΔnisA pnisA^L-nsoA1IE; ▲, FI5876ΔnisA pnisA^L-nsoA1YK; ♦, FI5876ΔnisA pnisA^L-nsoA4; □, FI5876ΔnisA pUK200. Results are the mean of triplicate measurements ±SD. (b) SEM (left) and TEM (right) analysis of the effect of hybrid construct expression on cell phenotype. Bar, 1 µm. (c) Growth of FI5876ΔnisA (▲), FI5876ΔnisP (●), FI5876ΔnisC (♦) and FI5876ΔnisB (■) containing pnisA^L-nsoA1YK (closed symbols) or pUK200 (open symbols). X, FI5876. (d) Viability of stationary-phase L. lactis FI5876 and knockout strains containing pnisA^L-nsoA1YK (red) or pUK200 (blue).