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Heterologous expression of the nso cluster. SDS-PAGE analysis (top) and Western hybridization (bottom) using the NsoA1 leader antibody. Comparison of L. lactis TCA-precipitated culture supernatant extracts from MG1614-pnsoΔnsoA (lanes 1 to 5) or MG1614-pnso (lanes 6–10) containing plasmids pTG262Pn (1 and 6), pTGnsoA1 (2 and 7) pTGnsoA2 (3 and 8), pTGnsoA3-nsoA4 (4, 5, 9 and 10) and cell extracts from B. obeum A2-162 (11) and FI5876 (12). M, marker.
