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. 2018 Mar 14;14(3):e1006916. doi: 10.1371/journal.ppat.1006916

Fig 5. Effect of TACSTD2 gene silencing on HCV infection in parental Huh7.5 cells.

Fig 5

(A-D) Parental Huh7.5 cells were transfected with either siControl or siTACSTD2 for 48h before infection with HCV. Cells were fixed with methanol either at 24h (A, B) or 48h post-infection (C, D) and visualized by immunofluorescence for CLDN1 (red)/HCV core (green) or OCLN (red)/HCV core (green) staining. (E) Relative levels of HCV infection at 24h and at 48h post-infection as shown by the semiquantitative representation of HCV core positive cells in parental Huh7.5 cells. Data represent the normalized mean ± SEM of the HCV core fluorescence intensity of triplicate experiments. A significant difference in HCV core fluorescence intensity between siControl- and siTACSTD2-treated cells was detected by paired t-test both at 24h and at 48h post-infection.