Fig 2. The agonistic activity of evocalcet on hCaR-expressing HEK293 (hCaR-HEK293) cells.

The transient changes in the cytoplasmic calcium concentration (Δ[Ca²⁺]i) induced by receptor activation were measured. (A) The effect of evocalcet on the Δ[Ca²⁺]i value in hCaR-HEK293 cells. (B) The effect of evocalcet on the response rates elicited by increasing the extracellular calcium level in hCaR-HEK293 cells. The response rate was calculated using the following formula:

$$\mathrm{R}\mathrm{e}\mathrm{s}\mathrm{p}\mathrm{o}\mathrm{n}\mathrm{s}\mathrm{e}\mathrm{r}\mathrm{a}\mathrm{t}\mathrm{e}\left(\mathrm{\%}\right)=\mathrm{\Delta }\left[{\mathrm{C}\mathrm{a}}^{2+}\right]\mathrm{i}/\mathrm{M}\mathrm{a}\mathrm{x}\mathrm{\Delta }\left[{\mathrm{C}\mathrm{a}}^{2+}\right]\mathrm{i}\times 100$$

Δ[Ca²⁺]i: Subtraction of basal [Ca²⁺]i from maximum [Ca²⁺]i observed after addition of evocalcet; Max Δ[Ca²⁺]i: Δ[Ca²⁺]i at the maximum Ca concentration (3 mmol/L).