Table 1. Quorum-sensing induced ICEMcSym1271 conjugative transfer.
| abDonor | Recipient | Exconjugants (per donor) | Standard deviation | cFold-change |
|---|---|---|---|---|
| WSM1271 | R7ANS(pPR3) | 8.02 x 10−8 | 1.82 x 10−8 | - |
| WSM1271(pSDz) | R7ANS(pPR3) | 2.22 x 10−8 | 9.12 x 10−9 | - |
| WSM1271(pSDz-traR1) | R7ANS(pPR3) | 4.69 x 10−7 | 1.11 x 10−7 | 21.14 |
| WSM1271(pSDz-traR2) | R7ANS(pPR3) | 5.97 x 10−7 | 1.66 x 10−7 | 26.90 |
| WSM1271(pSDz-msi172171) | R7ANS(pPR3) | 8.49 x 10−7 | 8.23 x 10−8 | 38.30 |
| WSM1271 | R7ANS(pFAJ1708) | 8.35 x 10−8 | 4.87 x 10−8 | - |
| WSM1271(pPR3) | R7ANS(pFAJ1708) | 8.74 x 10−8 | 3.89 x 10−8 | - |
| WSM1271(pPR3-traI1) | R7ANS(pFAJ1708) | 1.04 x 10−5 | 1.50 x 10−6 | 119.06 |
a Where appropriate, plasmids carried by WSM1271 are listed in brackets after the strain name (see Table 3 for a description of plasmids).
b Expression from the IPTG inducible promoter of pSDz constructs were not induced with IPTG as they exhibit leaky expression without induction in TY medium used for assays.
c Fold-change is relative to control strains carrying the appropriate pPR3 or pSDz parent vector.