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. 2018 Mar 15;27(6):391–404. doi: 10.1089/scd.2017.0268

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© Alexander Kondrashov et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — Polymorphic changes to the ADRB2 locus in hPSCs. The nucleotide and translated single-letter amino acid sequences are shown for the 5′ region of the ADRB2 locus. The targeting strategy introduces changes at positions 46 and 79 (nonsynonymous in the peptide), and 124 and 126 (synonymous in the peptide) as indicated. Features identified are the location of the gRNA underlined, with PAM site boxed, and TTAA PiggyBac cassette insertion site. PAM, protospacer adjacent motif. Color images available online at www.liebertpub.com/scd