FIG. 6.

Retention of pluripotency characteristics in the edited hPSC lines. (A–C) Show assessment of pluripotency characteristics in undifferentiated cells from each of the edited lines. This included (A) immunostaining for the transcription factor, OCT4 [green; inset with DAPI (blue) counterstaining], (B) flow cytometry for TRA-1-81 (blue), SSEA4 (green), and SSEA1 (red), relative to unstained (purple), and (C) G-banding karyotyping of 30 metaphase spreads per line, with a representative karyogram shown for each. In (D), directed monolayer differentiation produced CMs of >88% purity, as gauged by immunostaining for α-actinin (red) relative to total nuclei count (DAPI, blue). Scale bar is 100 μm; n = 2–4, SD. Color images available online at www.liebertpub.com/scd