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. Author manuscript; available in PMC: 2018 Apr 3.
Published in final edited form as: Biomed Microdevices. 2012 Feb;14(1):45–53. doi: 10.1007/s10544-011-9584-9

Figure 5.

Figure 5

Image of an MRSA v4.0 array through the film of a completely-intact plastic flow cell following on-chip amplification of 105 genomic copies of MRSA using PCR primers for mecA and tufA. The flow cell was washed with 35μl of 1X SSPE without implementing a drying protocol, and imaged for 0.5 sec with a GenePix 4000B. Colored circles correspond to different probes: Green, positive control Cy3 labeled oligos; purple, mecA gene, which confers methicillin resistance; blue, tufA gene found in S. Aureus strains; orange, tufA gene found in all Staph strains; red, nonsense spots that serve as a control for washing.