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. Author manuscript; available in PMC: 2018 Apr 3.
Published in final edited form as: Biomed Microdevices. 2012 Feb;14(1):45–53. doi: 10.1007/s10544-011-9584-9

Figure 6.

Figure 6

Limit of detection study using the MRSA v4.0 array along with on-chip amplification and pipet washing and drying protocol with intact plastic flow cells. Signal-to-nonsense ratios greater than 3 are considered positive. Nonsense signals indicate the level of biological noise due to nonspecific hybridization events. Images are acquired with Akonni’s Dx2000 reader, and ratios are determined with the use of Spotfinder, which calculates the integral spot intensity minus the local background of the targets spots and nonsense spots.