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. 2018 Feb 5;8(3):e2715. doi: 10.21769/BioProtoc.2715

Figure 1. Examples of representative endpoints during an extraction.

Figure 1.

A. An example of a cell pellet extraction to which 300 μl of 2:1 Methanol:Chloroform was added and vortexed. B. An example of a cell extraction after sonication (Step A6a.iii). C. The aqueous (1) and organic (2) layers are shown at the end of centrifugation (Steps A10 to A11).